Mining for Restriction Endonucleases in Nicaragua
نویسندگان
چکیده
منابع مشابه
The type IIB restriction endonucleases.
The endonucleases from the Type IIB restriction-modification systems differ from all other restriction enzymes. The Type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex. The fact that ...
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Prokaryotic restriction-modification (R-M) systems defend the host cell from the invasion of a foreign DNA. They comprise two enzymatic activities: specific DNA cleavage activity and DNA methylation activity preventing cleavage. Typically, these activities are provided by two separate enzymes: a DNA methyltransferase (MTase) and a restriction endonuclease (RE). In the absence of a corresponding...
متن کاملRecognition Sequences in the Restriction Endonucleases
The nature of specific protein-nucleic acid interaction between restriction endonucleases (RE) and their recognition sequences (RS) was studied by bioinformatics methods. It was found that the frequency of 5-6 residue long RS-like oligonucleotides is unexpectedly high in the nucleic acid sequence of the corresponding RE (p<0.05 and p<0.001 respectively, n=7). There is an extensive conservation ...
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The clastogenic ability of the restriction endonucleases (MspI, HpaII and HaeIII) in germinating seeds of reconstructed barley karyotype was assessed. An effective induction of chromosomal aberrations after restrictase treatment was observed. The frequency, types and cell-cycle dependence of the observed abnormalities are discussed in relation to the distinct characteristics of the enzymes and ...
متن کاملRestriction endonucleases and modification methylases in bacteria.
There are three mechanisms by which foreign DNA may enter a prokaryotic organism: conjugation between Gram-negative bacteria, uptake of exogenous DNA (transformation) and infection by viral (bacteriophage) nucleic acid. The interaction between a bacteriophage and its host bacterial cell has been extensively investigated. A bacterium can restrict phage infection through its ability to degrade th...
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ژورنال
عنوان ژورنال: Encuentro
سال: 2012
ISSN: 2220-7112,0424-9674
DOI: 10.5377/encuentro.v0i93.910